Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinase II

Biochem Biophys Res Commun. 1994 Dec 30;205(3):1776-84. doi: 10.1006/bbrc.1994.2875.

Abstract

Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. We have examined here whether Rabphilin-3A is phosphorylated by calmodulin-dependent protein kinase II (CaMKII). Recombinant Rabphilin-3A was phosphorylated by CaMKII purified from rat brain. Two moles of phosphate were maximally incorporated into one mole of Rabphilin-3A. The phosphorylation sites were Ser34, Thr205, Thr209, and Thr537. These results suggest that the CaMKII-catalyzed phosphorylation of Rabphilin-3A may modulate neurotransmitter release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Binding Sites / genetics
  • Brain / enzymology
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Cell-Free System
  • Cloning, Molecular
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism*
  • In Vitro Techniques
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Neurotransmitter Agents / metabolism
  • Phosphorylation
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Spodoptera
  • Vesicular Transport Proteins
  • rab GTP-Binding Proteins*

Substances

  • Adaptor Proteins, Signal Transducing
  • Nerve Tissue Proteins
  • Neurotransmitter Agents
  • Recombinant Proteins
  • Vesicular Transport Proteins
  • rabphilin-3A
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases
  • GTP-Binding Proteins
  • rab GTP-Binding Proteins

Associated data

  • GENBANK/U12571