Production of interleukin-10 by mouse glial cells in culture

Biochem Biophys Res Commun. 1994 Dec 30;205(3):1907-15. doi: 10.1006/bbrc.1994.2893.


In this study, we examined the production of IL-10 by glial cells in vitro. IL-10 was detected in the culture supernatants of microglia and in the cell lysate of astrocytes by enzyme-linked immunosorbent assay. We also detected IL-10 mRNA and IL-10 receptor mRNA in both microglia and astrocytes. The expression of IL-10 mRNA, as well as the production of IL-10 protein, was enhanced by the stimulation of these cells with lipopolysaccharide(LPS). Recombinant IL-10 effectively suppressed both LPS-induced cytokine production and IFN-gamma-induced class II major histocompatibility complex antigen expression by microglia. These results suggest that IL-10 is produced in the CNS and plays a role as an inhibitory regulator in the CNS cytokine network.

MeSH terms

  • Animals
  • Astrocytes / drug effects
  • Astrocytes / metabolism
  • Base Sequence
  • Cells, Cultured
  • Central Nervous System / metabolism
  • Cytokines / biosynthesis
  • DNA Primers / genetics
  • Interleukin-10 / biosynthesis*
  • Interleukin-10 / genetics
  • Lipopolysaccharides / pharmacology
  • Mice
  • Microglia / drug effects
  • Microglia / metabolism
  • Molecular Sequence Data
  • Neuroglia / drug effects
  • Neuroglia / metabolism*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin-10


  • Cytokines
  • DNA Primers
  • Lipopolysaccharides
  • RNA, Messenger
  • Receptors, Interleukin
  • Receptors, Interleukin-10
  • Interleukin-10