The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogues

Plant Mol Biol. 1994 Nov;26(4):1055-64. doi: 10.1007/BF00040688.


The octopine synthase (ocs or ocs-like) element has been previously reported to be responsive to the plant hormones, auxin, salicylic acid, and methyl jasmonate. Using transient assays with carrot protoplasts, we have demonstrated that an ocs element from the soybean auxin-inducible GH2/4 promoter is not only activated by strong auxins (i.e., 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, alpha-naphthalene acetic acid) and salicylic acid, but also by weak auxin analogues (beta-naphthalene acetic acid), inactive auxin analogs (i.e., 2,3-dichlorophenoxyacetic acid, 2,4,6-trichlorophenoxyacetic acid), and inactive salicylic acid analogs (3-hydroxybenzoic acid and 4-hydroxybenzoic acid). Our results indicate that the ocs element in the GH2/4 promoter is not selectively induced by plant hormones and might function similarly to tandem AP-1 sites in some animal glutathione S-transferase (GST) genes. The ocs element, like the AP-1 sites in animal GST promoters, may be induced not only by certain hormones but also by some non-hormonal stress-inducing or electrophilic agents.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Oxidoreductases / genetics*
  • Base Sequence
  • Daucus carota / genetics
  • Gene Expression Regulation, Plant*
  • Genes, Reporter
  • Indoleacetic Acids / pharmacology
  • Molecular Sequence Data
  • Plant Growth Regulators / pharmacology*
  • Promoter Regions, Genetic / genetics*
  • Salicylates / pharmacology
  • Soybeans / genetics*


  • Indoleacetic Acids
  • Plant Growth Regulators
  • Salicylates
  • Amino Acid Oxidoreductases
  • D-octopine dehydrogenase