A novel form of mouse cytochrome P450 3A (Cyp3a-16). Its cDNA cloning and expression in fetal liver

Eur J Biochem. 1994 Dec 15;226(3):877-82. doi: 10.1111/j.1432-1033.1994.t01-1-00877.x.

Abstract

A complementary DNA clone coding for a novel form of cytochrome P450, Cyp3a-16, in mouse fetal livers was isolated and completely sequenced. This clone encoded a polypeptide of 504 deduced amino acids and showed 87.3% and 66.6% amino acid identities with mouse Cyp3a-11 and Cyp3a-13, respectively. Cyp3a-16 transcript was detectable before birth and remarkably diminished five weeks after birth in mice. We conclude that Cyp3a-16 is a fetal- and puberty-specific cytochrome P450 in mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • Cytochrome P-450 CYP2E1
  • Cytochrome P-450 Enzyme System / chemistry
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification*
  • Gene Expression*
  • Humans
  • Liver / embryology*
  • Liver / enzymology*
  • Mice
  • Mice, Inbred ICR
  • Mixed Function Oxygenases / chemistry
  • Mixed Function Oxygenases / genetics*
  • Molecular Sequence Data
  • RNA, Messenger / metabolism

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • Cytochrome P-450 CYP2E1

Associated data

  • GENBANK/D16363
  • GENBANK/D26137
  • GENBANK/X63023