Abstract
Calcium entry, via a dihydropyridine-sensitive pathway, is required for differentiation in murine erythroleukemia cells (MELC). Calcium channel currents have been identified physiologically in some non-excitable cells, but little is known regarding the structure of these channels. We show that a truncated form of the alpha 1 subunit of the cardiac voltage-gated calcium channel (dihydropyridine receptor, DHPR) is expressed in MELC. This MELC calcium channel lacks the first four transmembrane segments of the DHPR (IS1 to IS4). A MELC calcium channel/cardiac DHPR chimera, co-expressed with the alpha 2 and beta subunits of the DHPR, forms a functional calcium channel in Xenopus oocytes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetamides / pharmacology
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Western
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Calcium Channels / genetics*
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Calcium Channels / metabolism
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Calcium Channels, L-Type
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Cell Differentiation / drug effects
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Cell Differentiation / genetics
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Cloning, Molecular
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DNA, Complementary
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Dihydropyridines / metabolism
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Leukemia, Erythroblastic, Acute / metabolism
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Mice
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Molecular Sequence Data
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Muscle Proteins / genetics*
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Muscle Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
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Xenopus laevis
Substances
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Acetamides
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Calcium Channels
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Calcium Channels, L-Type
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DNA, Complementary
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Dihydropyridines
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Muscle Proteins
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RNA, Messenger
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1,4-dihydropyridine
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hexamethylene bisacetamide