Selective permissiveness of TPA differentiated THP-1 myelomonocytic cells for human cytomegalovirus strains AD169 and Towne

Microb Pathog. 1994 May;16(5):373-8. doi: 10.1006/mpat.1994.1037.

Abstract

Two highly passaged laboratory strains of human cytomegalovirus (HCMV), AD169 and Towne, were tested for their ability to infect and replicate in THP-1 myelomonocytic cells differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment of human THP-1 cells increased the number of cells that expressed HCMV immediate early (IE1) antigen from 0.06% prior to TPA treatment to 12% following cell differentiation. The Towne but not the AD169 strain replicated in differentiated THP-1 cells as determined by HCMV DNA replication and infectious virus production. Major early (HCMVTRL4) mRNA was present in both the abortive and productive THP-1 cell infections.

Publication types

  • Comparative Study

MeSH terms

  • Cell Differentiation / drug effects
  • Cytomegalovirus / classification
  • Cytomegalovirus / growth & development
  • Cytomegalovirus / physiology*
  • DNA Replication / drug effects
  • DNA, Viral / biosynthesis
  • Gene Expression Regulation, Viral / drug effects
  • Humans
  • Leukemia, Myelomonocytic, Acute / pathology*
  • Monocytes / virology*
  • RNA, Messenger / analysis
  • RNA, Viral / analysis
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Tumor Cells, Cultured / virology
  • Virus Cultivation
  • Virus Replication*

Substances

  • DNA, Viral
  • RNA, Messenger
  • RNA, Viral
  • Tetradecanoylphorbol Acetate