F-actin binding site masked by the intramolecular association of vinculin head and tail domains

Nature. 1995 Jan 19;373(6511):261-4. doi: 10.1038/373261a0.

Abstract

Although vinculin is present at all sites of F-actin attachment to plasma membranes and is required for linkage of myofibrils to sarcolemma, it is unclear how it promotes attachment of actin to membranes. Because biochemical evidence for a direct interaction of vinculin with F-actin is controversial, current models of actin-membrane linkages depict only an indirect role for vinculin, as a tether for alpha-actinin. We demonstrate here that an intramolecular association between the 95K head and 30K tail domains of vinculin masks an F-actin binding site present in the carboxy-terminal tail domain. Cosedimentation and crosslinking assays, and direct visualization by transmission electron microscopy, reveal an interaction between F-actin and a bacterially expressed fusion protein containing amino acids 811-1066 of vinculin, and between F-actin and a proteolytic fragment of vinculin containing amino acids 858-1066. Vinculin itself neither cosediments with nor crosslinks F-actin. The amino-terminal 95K head fragment of vinculin, but not intact vinculin, inhibits both cosedimentation and crosslinking. We propose that assembly of vinculin into an adherens junction involves disruption of the head-tail interaction, revealing a site that mediates microfilament attachment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism*
  • Actins / ultrastructure
  • Animals
  • Binding Sites
  • Cell Membrane / metabolism
  • Chickens
  • Glutathione Transferase / metabolism
  • Peptide Fragments / metabolism
  • Rabbits
  • Recombinant Fusion Proteins
  • Serine Endopeptidases
  • Vinculin / metabolism*
  • Vinculin / ultrastructure

Substances

  • Actins
  • Peptide Fragments
  • Recombinant Fusion Proteins
  • Vinculin
  • Glutathione Transferase
  • Serine Endopeptidases
  • glutamyl endopeptidase