Practical guidance for testing the accuracy of deconvolution results from quantal analysis

Pflugers Arch. 1994 Oct;428(3-4):418-21. doi: 10.1007/BF00724527.

Abstract

A Monte Carlo study was carried out to test the reliability of the Maximum Likelihood Estimator (MLE) approach for quantal analysis. This widely used statistical method was applied to extract a finite mixture of Gaussian distributions from simulated data. The data were generated by convolving a distribution of discrete amplitude steps (multiples of a unitary step Q) with Gaussian noise of various standard deviations (sigma n). Our results offer practical guidance on when to use the MLE, taking into account the determining parameters: signal to noise ratio (Q/sigma n, the most important parameter), number of samples collected and the number of components (k). For a given set of parameters the algorithm always converged to the "true" values, never converged to the "true" values or converged in only a fraction of cases to the "true" values. The behavior of the fitting routine in the parameter space is displayed in contour plots. These contour plots can be used as a guide to test the accuracy of deconvolution results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computer Simulation
  • Models, Neurological*
  • Monte Carlo Method