A human orphan calcitonin receptor-like structure

Biochem Biophys Res Commun. 1995 Jan 5;206(1):341-7. doi: 10.1006/bbrc.1995.1047.

Abstract

A novel calcitonin receptor-like protein of 461 amino acids with seven putative transmembrane domains has been identified through molecular cloning in a cDNA library of the human cerebellum. 91% and 56% of the amino acids are identical in a rat orphan calcitonin receptor-like sequence and the human calcitonin receptor, respectively. 5.2 kb mRNA is predominantly expressed in the lung, heart and kidney. Specific binding of 125I-labeled salmon calcitonin and human calcitonin gene-related peptide-I to COS-7 cells transiently transfected with the receptor cDNA was less then 0.5%. Cellular cAMP accumulation was indistinguishable in cDNA transfected and non-transfected control COS-7 and renal tubular cells from the American opossum stimulated with human and salmon calcitonin, human calcitonin gene-related peptide-I and -II, human amylin, human adrenomedullin, lizard helodermin, salmon stanniocalcin and chicken parathyroid hormone-related protein. The receptor-like protein whose ligand remains to be discovered belongs to the family of receptors of calcitonin, parathyroid hormone, secretin, vasointestinal peptide and pituitary adenylate cyclase-activating polypeptide.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Calcitonin / metabolism*
  • Calcitonin / pharmacology
  • Cell Line
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Cyclic AMP / metabolism
  • DNA, Complementary
  • Female
  • Gene Expression
  • Humans
  • Kidney / metabolism
  • Kidney Tubules / drug effects
  • Kidney Tubules / metabolism
  • Lung / metabolism
  • Molecular Sequence Data
  • Myocardium / metabolism
  • Opossums
  • Organ Specificity
  • Phylogeny
  • Pregnancy
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, Calcitonin / biosynthesis*
  • Receptors, Calcitonin / chemistry
  • Salmon
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Calcitonin
  • Calcitonin
  • Cyclic AMP

Associated data

  • GENBANK/U17473