A soluble cellular factor directly stimulates Ca2+ entry in neutrophils

Biochem Biophys Res Commun. 1995 Jan 5;206(1):348-54. doi: 10.1006/bbrc.1995.1048.

Abstract

A soluble factor, extracted from neutrophils and P388D1 cells, stimulated a transient rise in cytosolic free Ca2+ and a small increase in the permeability to Mn2+ in fura2-loaded neutrophils. These effects were not prevented by blockade of formylated peptide receptors by t-boc-met-leu-phe. The rise in cytosolic free Ca2+ was partly attributed to transmembrane influx and partly due to store release. Ca2+ store release but not transmembrane influx was inhibited by the PLC inhibitor, U73122, demonstrating a direct effect of the factor on channel opening. It was concluded that the soluble cellular factor directly stimulated Ca2+ entry in neutrophils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Factors / isolation & purification
  • Biological Factors / pharmacology*
  • Calcium / blood*
  • Cytosol / metabolism
  • Estrenes / pharmacology
  • Fura-2
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Leukemia P388
  • Mice
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / drug effects
  • Neutrophils / metabolism*
  • Neutrophils / physiology
  • Pyrrolidinones / pharmacology
  • Time Factors
  • Tumor Cells, Cultured
  • Type C Phospholipases / antagonists & inhibitors

Substances

  • Biological Factors
  • Estrenes
  • Pyrrolidinones
  • 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
  • N-Formylmethionine Leucyl-Phenylalanine
  • Type C Phospholipases
  • Calcium
  • Fura-2