Extraction and purification of a substance with luteinizing hormone releasing activity from the leaves of Avena sativa

Tohoku J Exp Med. 1976 Jun;119(2):115-22. doi: 10.1620/tjem.119.115.

Abstract

Attempts were made to purify the LH-releasing substance extracted from the leaves of Avena sativa by means of two-step chromatographic procedures using a weakly acidic ion-exchange resin (CG-50,type II) and DEAE-Sephadex A-25 (coarse) with successful results. For preliminary fractionation of such starting materials as dried leaves, fresh leaves, and acetone-extracted powder(crude extracts), 5% acetate-buffered active carbon proved to be more effective than starch zone electrophoresis. From its behavior on chromatography with weakly acidic ion-exchange resins as well as Sephadex gel filtration, the active fraction extracted from the leaves of Avena saliva was assumed to be different from the LH-RH present in the hypothalamus. This partially purified material, however, was demonstrated to have an LH-releasing activity by the ovarian ascorbic acid depletion method using Wistar-Imamichi strain rats. Evidence was presented that its site of action is in the adenohypophysis.

MeSH terms

  • Animals
  • Chromatography, DEAE-Cellulose
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Chromatography, Thin Layer
  • Edible Grain
  • Female
  • Gonadotropin-Releasing Hormone / isolation & purification*
  • Male
  • Plant Extracts / analysis
  • Plants / analysis*
  • Rats

Substances

  • Plant Extracts
  • Gonadotropin-Releasing Hormone