Contribution of mutant analysis to the understanding of enzyme catalysis: the case of class A beta-lactamases

Biochim Biophys Acta. 1995 Jan 19;1246(2):109-27. doi: 10.1016/0167-4838(94)00177-i.


Class A beta-lactamases represent a family of well studied enzymes. They are responsible for many antibiotic resistance phenomena and thus for numerous failures in clinical chemotherapy. Despite the facts that five structures are known at high resolution and that detailed analyses of enzymes modified by site-directed mutagenesis have been performed, their exact catalytic mechanism remains controversial. This review attempts to summarize and to discuss the many available data.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Catalysis*
  • DNA Mutational Analysis
  • Molecular Sequence Data
  • Molecular Structure
  • Substrate Specificity
  • beta-Lactamases / genetics*


  • beta-Lactamases