Construction of a human full-length cDNA bank

Gene. 1994 Dec 15;150(2):243-50. doi: 10.1016/0378-1119(94)90433-2.


We aimed to construct a full-length cDNA bank from an entire set of human genes and to analyze the function of a protein encoded by each cDNA. To achieve this purpose, a multifunctional phagemid shuttle vector, pKA1, was constructed for preparing a high-quality cDNA library composed of full-length cDNA clones which can be sequenced and expressed in vitro and in mammalian cells without subcloning the cDNA fragment into other vectors. Using this as a vector primer, we have prepared a prototype of the bank composed of full-length cDNAs encoding 236 human proteins whose amino acid sequences are identical or similar to known proteins. Most cDNAs contain a putative cap site sequence, some of which show a pyrimidine-rich conserved sequence exhibiting polymorphism. It was confirmed that the vector permits efficient in vitro translation, expression in mammalian cells and the preparation of nested deletion mutants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary*
  • Gene Expression
  • Gene Library*
  • Genetic Vectors
  • Hominidae / genetics*
  • Humans
  • Mammals
  • Molecular Sequence Data
  • Proteins / genetics*
  • Restriction Mapping


  • DNA, Complementary
  • Proteins

Associated data

  • GENBANK/D13749
  • GENBANK/D28342
  • GENBANK/D28343
  • GENBANK/D28344
  • GENBANK/D28345
  • GENBANK/D28346
  • GENBANK/D28347
  • GENBANK/D28348
  • GENBANK/D28349
  • GENBANK/D28350
  • GENBANK/D28351
  • GENBANK/D28352
  • GENBANK/D28353
  • GENBANK/D28354
  • GENBANK/D28355
  • GENBANK/D28356
  • GENBANK/D28357
  • GENBANK/D28358
  • GENBANK/D28359
  • GENBANK/D28360
  • GENBANK/D28361
  • GENBANK/D28362
  • GENBANK/D28363
  • GENBANK/D28364
  • GENBANK/D28365
  • GENBANK/D28366
  • GENBANK/D28367
  • GENBANK/D28368
  • GENBANK/D28369
  • GENBANK/D28370