Identification and characterization of a cDNA encoding ribosomal protein S12 from Xenopus laevis

Gene. 1994 Dec 15;150(2):331-3. doi: 10.1016/0378-1119(94)90447-2.

Abstract

The complete nucleotide (nt) sequence of the cDNA clone XL-S12, encoding a Xenopus laevis (Xl) homologue of the mammalian ribosomal protein S12, has been determined. The sequence predicts a Xl S12 protein of 132 amino acids (aa) with a molecular mass of 14.7 kDa. Xl S12 shares 95 and 97% aa sequence identity with the human and murine S12 proteins, respectively. Analysis of nt substitution patterns and rates indicates that S12 is a very highly constrained protein, evolving at an estimated rate of only 0.03 x 10(-9) non-synonymous (protein-altering) substitutions per site per year.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution*
  • DNA, Complementary / analysis*
  • DNA, Complementary / chemistry
  • Hominidae / genetics
  • Humans
  • Mammals
  • Mice / genetics
  • Molecular Sequence Data
  • Rats / genetics
  • Ribosomal Proteins / biosynthesis
  • Ribosomal Proteins / genetics*
  • Sequence Homology, Nucleic Acid
  • Xenopus laevis / genetics*

Substances

  • DNA, Complementary
  • Ribosomal Proteins
  • ribosomal protein S12

Associated data

  • GENBANK/L32600