Abstract
A 2618-bp cDNA that encodes the human mitochondrial glycerol-3-phosphate dehydrogenase has been isolated from a HeLa cell cDNA library and the nucleotide sequence determined. An open reading frame encodes a protein of 727 amino acids that is 96% similar to the rat protein and, like the rat protein, contains sites homologous to the Ca(2+)-binding sites of calmodulin, as well as FAD- and putative glycerol-phosphate-binding sites.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Calcium / metabolism
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Calmodulin / metabolism
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DNA, Complementary
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Flavin-Adenine Dinucleotide / metabolism
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Gene Library
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Glycerolphosphate Dehydrogenase / genetics*
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Glycerophosphates / metabolism
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HeLa Cells
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Hominidae / genetics*
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Humans
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Molecular Sequence Data
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Open Reading Frames
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Rats
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Sequence Homology, Amino Acid
Substances
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Calmodulin
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DNA, Complementary
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Glycerophosphates
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Flavin-Adenine Dinucleotide
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Glycerolphosphate Dehydrogenase
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Calcium