Intraperitoneal injections of Fluorogold reliably labels all sympathetic preganglionic neurons in the rat

J Neurosci Methods. 1994 Aug;53(2):137-41. doi: 10.1016/0165-0270(94)90170-8.

Abstract

The ability of intraperitoneal injections of a retrograde neuronal tracer, Fluorogold, to label the entire population of sympathetic preganglionic neurones was tested with a double-labelling strategy. Animals were injected intraperitoneally (i.p.) with Fluorogold, while Fast Blue or subunit B of cholera toxin were injected into a peripheral autonomic ganglion or into the adrenal gland. Sympathetic preganglionic neurones were then examined for retrogradely transported tracers. In all cases, preganglionic neurones labelled with Fast Blue or cholera toxin also contained Fluorogold, indicating that i.p. injections of Fluorogold do reliably label the entire population of sympathetic preganglionic neurones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Glands / innervation
  • Adrenergic Fibers / metabolism*
  • Amidines
  • Animals
  • Autonomic Fibers, Preganglionic / metabolism*
  • Cholera Toxin
  • Fluorescent Dyes*
  • Histocytochemistry / methods*
  • Injections, Intraperitoneal
  • Male
  • Microscopy, Fluorescence
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord / cytology
  • Stilbamidines*

Substances

  • 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt
  • Amidines
  • Fluorescent Dyes
  • Stilbamidines
  • diamidino compound 253-50
  • Cholera Toxin