Alternative form of the dicluster ferredoxin from the thermoacidophilic archaeon, Sulfolobus sp. strain 7

Biochem Biophys Res Commun. 1995 Jan 17;206(2):563-9. doi: 10.1006/bbrc.1995.1080.


An alternative form of a 7Fe dicluster-type ferredoxin (Fd-B) with a different charge density was purified as a minor component from the aerobic and thermoacidophilic archaeon, Sulfolobus sp. strain 7. Comparison of its properties with those of native 7Fe ferredoxin (Fd-A), a major ferredoxin, was made in terms of the molecular properties, the absorption, circular dichroism and electron paramagnetic resonance spectral properties, and the reactivity coupled with the cognate 2-oxoacid:ferredoxin oxidoreductase at 50 degrees C. Our results suggest that the Sulfolobus 7Fe ferredoxin has two spectroscopically distinct forms with slightly different conformations.

Publication types

  • Comparative Study

MeSH terms

  • Aerobiosis
  • Amino Acid Sequence
  • Chromatography, Ion Exchange
  • Circular Dichroism
  • Electron Spin Resonance Spectroscopy
  • Electrophoresis, Polyacrylamide Gel
  • Ferredoxins / chemistry*
  • Ferredoxins / isolation & purification
  • Ferredoxins / metabolism*
  • Hot Temperature
  • Iron / analysis
  • Ketone Oxidoreductases / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Protein Conformation*
  • Sequence Homology, Amino Acid
  • Sulfolobus / metabolism*


  • Ferredoxins
  • Iron
  • Ketone Oxidoreductases
  • 2-oxoglutarate synthase