Crystal structure of C-T-C-T-C-G-A-G-A-G. Implications for the structure of the Holliday junction

D S Goodsell; K Grzeskowiak; R E Dickerson

doi:10.1021/bi00003a037

Crystal structure of C-T-C-T-C-G-A-G-A-G. Implications for the structure of the Holliday junction

Biochemistry. 1995 Jan 24;34(3):1022-9. doi: 10.1021/bi00003a037.

Authors

D S Goodsell¹, K Grzeskowiak, R E Dickerson

Affiliation

¹ Department of Chemistry and Biochemistry, University of California, Los Angeles 90024.

PMID: 7827018
DOI: 10.1021/bi00003a037

Abstract

The structure of the B-DNA decamer of sequence C-T-C-T-C-G-A-G-A-G shows a crossed arrangement of helices in the C2 crystal lattice. This is the fourth example of a crossed arrangement of B-DNA oligomers in a crystal, and in spite of the fact that each of these four crystallizes in a different lattice, all have nearly identical structures at the crossing contact. This ubiquitous crossing arrangement may be used to generate a structure for the Holliday junction that is fully consistent with the available physical data.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Calcium / chemistry
Crystallography, X-Ray
DNA / chemistry*
Molecular Sequence Data
Nucleic Acid Conformation*
Oligodeoxyribonucleotides / chemistry*
Recombination, Genetic

Substances

Oligodeoxyribonucleotides
DNA
Calcium