Interleukin-6 functions as an autocrine growth factor in a cholangiocarcinoma cell line

J Gastroenterol Hepatol. Sep-Oct 1994;9(5):462-7. doi: 10.1111/j.1440-1746.1994.tb01275.x.

Abstract

The tumour cells of a human cholangiocarcinoma cell line, HuCC-T1, were found to express mRNA of interleukin-6 (IL-6) and to secrete a large amount of biologically active IL-6 in the culture medium at the concentration of 22.6 ng/mL. Interleukin-6 was demonstrated in the cytoplasm of the cells by immunohistochemical staining. Furthermore, these cells showed the presence of receptors for IL-6 on the surface, and DNA synthesis of the cells was stimulated by the exogenous addition of recombinant human IL-6 into the culture medium. The cell growth was significantly inhibited in the presence of anti-human IL-6 antibody in the culture medium. These findings indicate that IL-6 is one of the autocrine growth factors of this cell line in vitro.

MeSH terms

  • Cholangiocarcinoma / chemistry
  • Cholangiocarcinoma / metabolism*
  • Growth Substances / physiology*
  • Humans
  • Interleukin-6 / metabolism
  • Interleukin-6 / physiology*
  • Liver Neoplasms / chemistry
  • Liver Neoplasms / metabolism*
  • Receptors, Interleukin / analysis
  • Receptors, Interleukin-6
  • Tumor Cells, Cultured

Substances

  • Growth Substances
  • Interleukin-6
  • Receptors, Interleukin
  • Receptors, Interleukin-6