Identification of two cell cycle regulated genes affecting the beta 1,3-glucan content of cell walls in Saccharomyces cerevisiae

FEBS Lett. 1995 Jan 23;358(2):165-70. doi: 10.1016/0014-5793(94)01418-z.


The Calcofluor white-hypersensitive mutants cwh52 and cwh53 are severely reduced in beta 1,3-glucan. CWH52 was equivalent to GAS1. CWH53 represented a new gene, located on the right arm of chromosome XII, and predicted to encode a 215 kDa protein with multiple transmembrane domains. The transcription of CWH53 was cell cycle-dependent and, similar to GAS1/CWH52, increased in late G1, indicating that the formation of beta-glucan is cell cycle-regulated. Further, in some mutant alleles of both gas1/cwh52 and cwh53 lethal concentrations of Calcofluor induced growth arrest at a specific phase of the cell cycle.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle / genetics*
  • Cell Wall / metabolism*
  • Chromosomes, Fungal
  • Cloning, Molecular
  • Consensus Sequence
  • Gene Expression Regulation, Fungal*
  • Glucans / metabolism*
  • Molecular Sequence Data
  • Mutation
  • Oligodeoxyribonucleotides
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • beta-Glucans*


  • Glucans
  • Oligodeoxyribonucleotides
  • beta-Glucans
  • beta-1,3-glucan

Associated data

  • GENBANK/X80817