Cloning of a full-length cDNA encoding the neutrophil-activating peptide ENA-78 from human platelets

Gene. 1994 Dec 30;151(1-2):333-4. doi: 10.1016/0378-1119(94)90682-3.

Abstract

Here we describe the cloning of a full-length cDNA encoding a neutrophil chemoattractant peptide, ENA-78, from human platelets. The cDNA encodes a predicted sequence of 114 amino acids and contains the Cys motif C-X-C found in other members of the alpha-chemokine family which also includes interleukin 8 (IL-8). ENA-78 has a high degree of sequence identity with other platelet-derived chemokines which also share overlapping chemotactic activities such as GRO alpha and the neurophil-activating peptide 2 (NAP-2; derived by proteolytic cleavage of the connective-tissue-activating peptide III (CTAP-III)).

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blood Platelets / metabolism*
  • Chemokine CXCL5
  • Chemokines, CXC*
  • Chemotactic Factors / genetics
  • Cloning, Molecular / methods*
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / chemistry
  • Humans
  • Interleukin-8 / analogs & derivatives*
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Repetitive Sequences, Nucleic Acid

Substances

  • CXCL5 protein, human
  • Chemokine CXCL5
  • Chemokines, CXC
  • Chemotactic Factors
  • DNA, Complementary
  • Interleukin-8

Associated data

  • GENBANK/X78686