Isolation of nested, bidirectional deletions by the use of exonuclease III on double-origin vectors

Gene. 1994 Dec 30;151(1-2):97-101. doi: 10.1016/0378-1119(94)90637-8.

Abstract

When used in conjunction with double-origin vectors, the popular exonuclease III method can generate nested deletions from both ends of cloned DNA fragments. Such bidirectional deletions can be useful in sequencing long DNA fragments in both directions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA / chemistry*
  • DNA / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Exodeoxyribonucleases*
  • Genetic Techniques
  • Genetic Vectors*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / isolation & purification*
  • Restriction Mapping
  • Sequence Deletion*

Substances

  • Oligodeoxyribonucleotides
  • DNA
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III

Associated data

  • GENBANK/U13761
  • GENBANK/U13762