An Ordered NotI Fragment Map of Human Chromosome Band 11p15

Genomics. 1994 Sep 1;23(1):211-22. doi: 10.1006/geno.1994.1479.

Abstract

An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH-CALCA-ST5-RBTN1-HPX-HBB-RRM1 -TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS - RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cells, Cultured
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Cosmids
  • DNA Probes
  • Deoxyribonucleases, Type II Site-Specific*
  • Electrophoresis, Gel, Pulsed-Field
  • Humans
  • Hybrid Cells
  • Restriction Mapping*
  • Sequence Deletion

Substances

  • DNA Probes
  • Deoxyribonucleases, Type II Site-Specific
  • GCGGCCGC-specific type II deoxyribonucleases