Expression of PDGF and PDGF Receptors in Human Astrocytoma Operation Specimens Supports the Existence of an Autocrine Loop

Int J Cancer. 1995 Jan 17;60(2):168-73. doi: 10.1002/ijc.2910600206.

Abstract

Established cell lines derived from human malignant astrocytomas typically express a combination of platelet-derived growth factor (PDGF) and PDGF receptor which could form an autocrine loop. In this study, we screened for the essential components of a PDGF autocrine loop in fresh surgical isolates of human astrocytomas, using in situ hybridization and immunohistochemical techniques. Eight malignant astrocytomas (6 glioblastomas and 2 anaplastic astrocytomas), 5 low-grade astrocytomas and 4 non-neoplastic glial specimens (mesial temporal sclerosis) were evaluated. Malignant astrocytomas, and to a lesser extent low-grade astrocytomas, expressed more PDGF-A and PDGF-B than non-neoplastic glia. PDGF-alpha-receptor expression was elevated both in malignant and in low-grade astrocytomas. These data support the argument that PDGF autocrine loops contribute to the unregulated growth of human astrocytomas. Expression of PDGF and PDGF receptor in low-grade astrocytomas suggests that activation of PDGF autocrine loops may be an early event in the pathogenesis of malignant astrocytomas.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Astrocytoma / chemistry*
  • Child
  • Child, Preschool
  • Gene Rearrangement
  • Humans
  • Middle Aged
  • Platelet-Derived Growth Factor / analysis*
  • Receptors, Platelet-Derived Growth Factor / analysis*
  • Receptors, Platelet-Derived Growth Factor / genetics

Substances

  • Platelet-Derived Growth Factor
  • Receptors, Platelet-Derived Growth Factor