Direct in vivo evidence that D2 dopamine receptors can modulate dopamine uptake

Neurosci Lett. 1994 Aug 1;176(2):259-63. doi: 10.1016/0304-3940(94)90096-5.


In vivo electrochemistry was used to determine the effects of locally applied raclopride (a D2 receptor antagonist) and SCH-23390 (a D1 receptor antagonist) on the clearance of locally applied dopamine in the striatum, nucleus accumbens, and medial prefrontal cortex of rats. Chronoamperometric recordings were continuously made at 5 Hz using Nafion-coated, single carbon fiber electrodes. When a calibrated amount of dopamine was pressure ejected at 5-min intervals from a micropipette adjacent (280-310 microns) to the electrode, transient and reproducible dopamine signals were detected in all three regions. Local application of raclopride from a second micropipette, prior to pressure ejection of dopamine, increased the amplitude and time course of the dopamine signals, indicating significant inhibition of the dopamine transporter. In contrast, local application of SCH-23390 or saline had no effect on the dopamine signals. These data indicate that D2, but not D1, dopamine receptors can modulate the activity of the dopamine transporter.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Benzazepines / pharmacology
  • Brain / drug effects
  • Brain / metabolism*
  • Dopamine / metabolism*
  • Dopamine Antagonists / pharmacology
  • Dopamine D2 Receptor Antagonists
  • Electrochemistry
  • Male
  • Neostriatum / drug effects
  • Neostriatum / metabolism
  • Nucleus Accumbens / drug effects
  • Nucleus Accumbens / metabolism
  • Prefrontal Cortex / drug effects
  • Prefrontal Cortex / metabolism
  • Raclopride
  • Rats
  • Rats, Inbred F344
  • Receptors, Dopamine D1 / antagonists & inhibitors
  • Receptors, Dopamine D2 / metabolism*
  • Salicylamides / pharmacology


  • Benzazepines
  • Dopamine Antagonists
  • Dopamine D2 Receptor Antagonists
  • Receptors, Dopamine D1
  • Receptors, Dopamine D2
  • Salicylamides
  • Raclopride
  • Dopamine