Immunoscintigraphy of bone sarcomas--results in 5 patients

Eur J Cancer. 1994;30A(10):1484-9. doi: 10.1016/0959-8049(94)00304-n.


The feasibility of using the murine monoclonal antibody, TP-1, for clinical immunoscintigraphy was examined in a pilot study involving 5 patients with bone sarcomas. 131I-labelled F(ab')2 antibody fragments were injected in doses of 0.8-1.0 mg (90-130 MBq), and the accumulation of radioactivity was examined by scintigraphy, and assessed by direct measurements on biopsied tumour and normal tissue. One osteosarcoma patient had a primary tumour in the femur, whereas the other 4 had single lung metastases detected by other diagnostic methods. Immunoscintigraphy of the femoral primary was optimally visualised after 22 h. In 2 patients, the method failed to detect lung metastasis, in 1 of the cases possibly related to less than optimal methodological conditions. In 2 other patients, increased accumulation of radioactivity indicated one and three lung tumours, in addition to the single metastasis observed by X-ray and CT scanning, tumours that were later confirmed and removed surgically. The concentration of radioactivity in tumour and normal tissues 44-72 h after antibody injection could be measured in 4 patients. The tumour to blood ratios were in the range of 1.2-4.2, compared to 0.1-0.8 for various normal tissues. The results indicate that immunoscintigraphy with TP-1 antibody fragments have a potential for early detection of lung metastases in patients with bone sarcoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Monoclonal / blood
  • Bone Neoplasms / diagnostic imaging*
  • Femoral Neoplasms / diagnostic imaging*
  • Humans
  • Immunoglobulin Fab Fragments / blood
  • Iodine Radioisotopes
  • Lung Neoplasms / diagnostic imaging*
  • Lung Neoplasms / pathology
  • Lung Neoplasms / secondary*
  • Male
  • Middle Aged
  • Pilot Projects
  • Sarcoma / diagnostic imaging*
  • Sarcoma / pathology
  • Sarcoma / secondary*
  • Tomography, Emission-Computed, Single-Photon


  • Antibodies, Monoclonal
  • Immunoglobulin Fab Fragments
  • Iodine Radioisotopes