Early identification of Epstein-Barr virus-associated post-transplantation lymphoproliferative disease

Br J Haematol. 1995 Jan;89(1):98-103. doi: 10.1111/j.1365-2141.1995.tb08904.x.


Epstein-Barr virus-associated lymphoproliferative disease (EBV-LPD) is a common, usually fatal, complication developing after transplantation of bone marrow from HLA-mismatched or HLA-matched unrelated donors. Prompted by recent reports of successful treatment of EBV-LPD, we investigated methods which could result in early identification of patients at high risk for this disorder, thus improving the likelihood of successful therapeutic interventions. Both the outgrowth of transformed B lymphocytes ex vivo (100% correlation) and the detection of EBV DNA by a PCR method (80% correlation) showed statistically significant association with the histopathological diagnosis of EBV-LPD. Because these abnormalities can be detected prior to the onset of clinical disease. It should now be possible to use a combination of the methods described here to identify patients at high risk of developing EBV-LPD, thus enabling early therapeutic intervention.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • B-Lymphocytes / pathology
  • Blotting, Southern
  • Bone Marrow Transplantation / adverse effects*
  • Cell Division
  • Cell Transformation, Viral
  • Cells, Cultured
  • Child
  • Child, Preschool
  • DNA, Viral / blood
  • Female
  • Herpesviridae Infections / diagnosis*
  • Herpesvirus 4, Human / isolation & purification*
  • Humans
  • Leukemia, Myeloid / therapy
  • Lymphoproliferative Disorders / blood
  • Lymphoproliferative Disorders / diagnosis*
  • Lymphoproliferative Disorders / virology
  • Male
  • Time Factors
  • Tumor Virus Infections / diagnosis*


  • DNA, Viral