Dendritic cell progenitor is transformed by a conditional v-Rel estrogen receptor fusion protein v-RelER

Cell. 1995 Jan 27;80(2):341-52. doi: 10.1016/0092-8674(95)90417-4.


A conditional v-Rel estrogen receptor fusion protein, v-RelER, causes estrogen-dependent but otherwise unaltered v-rel-specific transformation of chicken bone marrow cells. Here, we demonstrate that such v-relER-transformed cells exhibit B lymphoid determinants in line with earlier studies on v-rel-transformed cells. However, following inactivation of v-RelER oncoprotein activity by administration of an estrogen antagonist, cells differentiate into antigen-presenting dendritic cells as judged by several morphological and functional criteria. Additionally, under yet different culture conditions, v-relER cells differentiate into cells resembling polymorphonuclear neutrophils. Our studies therefore suggest that the conditional v-RelER, and probably also the authentic v-Rel, transform a common progenitor for neutrophils and dendritic cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / immunology
  • Bone Marrow
  • Cell Differentiation
  • Cell Line, Transformed
  • Chick Embryo
  • Dendritic Cells / physiology*
  • Dendritic Cells / ultrastructure
  • Fibroblasts
  • Flow Cytometry
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / physiology*
  • Hematopoietic Stem Cells / ultrastructure
  • Lymphocyte Culture Test, Mixed
  • Macrophages
  • Microscopy, Electron
  • Neutrophils / cytology*
  • Neutrophils / physiology
  • Oncogene Proteins v-rel
  • Oncogenes*
  • Phagocytosis
  • Receptors, Estrogen / biosynthesis*
  • Recombinant Fusion Proteins / biosynthesis
  • Retroviridae Proteins, Oncogenic / biosynthesis*
  • T-Lymphocytes / immunology
  • Transcription Factors / biosynthesis
  • Vimentin / biosynthesis


  • Oncogene Proteins v-rel
  • Receptors, Estrogen
  • Recombinant Fusion Proteins
  • Retroviridae Proteins, Oncogenic
  • Transcription Factors
  • Vimentin