The gingiva samples were obtained from 40 patients with chronic periodontitis after flap operation and 20 cases having healthy gingiva after ectopic wisdom tooth extraction. We investigated the oxytalan fibers by light microscopy (LM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The results demonstrated that under normal conditions numerous oxytalan fibers were widely and evenly distributed throughout the periodontal tissues. There was marked degeneration of the oxytalan fibers in chronic peridontitis. The predominant features were disintegration, fragmentation or disappearance of the oxytalan fibers under the basement membrane. Around the blood vessels, the oxytalan fibers decreased in density with fragmentation of the fibers and sometimes they disappeared almost completely. These results indicated that degeneration and destruction of the oxytalan fibers were closely correlated with the chronic periodontitis process, leading to reduced function occlusion of teeth.