Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jan 16;14(2):217-23.

Transport Route for Synaptobrevin via a Novel Pathway of Insertion Into the Endoplasmic Reticulum Membrane

Affiliations
Free PMC article

Transport Route for Synaptobrevin via a Novel Pathway of Insertion Into the Endoplasmic Reticulum Membrane

U Kutay et al. EMBO J. .
Free PMC article

Abstract

Synaptobrevin/vesicle-associated membrane protein is one of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. It is proposed to provide specificity for the targeting and fusion of vesicles with the plasma membrane. It belongs to a class of membrane proteins which lack a signal sequence and contain a single hydrophobic segment close to their C-terminus, leaving most of the polypeptide chain in the cytoplasm (tail-anchored). We show that in neuroendocrine PC12 cells, synaptobrevin is not directly incorporated into the target organelle, synaptic-like vesicles. Rather, it is first inserted into the endoplasmic reticulum (ER) membrane and is then transported via the Golgi apparatus. Its insertion into the ER membrane in vitro occurs post-translationally, is dependent on ATP and results in a trans-membrane orientation of the hydrophobic tail. Membrane integration requires ER protein(s) different from the translocation components needed for proteins with signal sequences, thus suggesting a novel mechanism of insertion.

Similar articles

See all similar articles

Cited by 97 articles

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1994 May 24;91(11):4688-92 - PubMed
    1. J Biol Chem. 1978 Nov 25;253(22):8203-9 - PubMed
    1. Cell. 1992 Oct 30;71(3):489-503 - PubMed
    1. J Cell Biol. 1987 Feb;104(2):201-8 - PubMed
    1. Nature. 1993 Mar 25;362(6418):318-24 - PubMed

Publication types

LinkOut - more resources

Feedback