Heterogeneity of liver-kidney microsomal autoantibodies in chronic hepatitis C and D virus infection

Gastroenterology. 1995 Feb;108(2):455-62. doi: 10.1016/0016-5085(95)90074-8.


Background/aims: Anti-liver-kidney microsomal (LKM) autoantibodies occur in a proportion of patients with chronic hepatitis C and D infections. Because of different immunofluorescence patterns, antibodies in hepatitis C and D were termed LKM-1 and LKM-3, respectively. The aim of the present study was to evaluate the different specificities of LKM-1 and LKM-3 antibodies.

Methods: Forty-nine samples of LKM-1 sera and 16 samples of LKM-3 sera were studied for reactivity against rat and human liver microsomal proteins by immunofluorescence, enzyme-linked immunosorbent assay, and Western blot.

Results: Thirty-four percent of the LKM-1 sera reacted with 50-kilodalton cytochrome P4502D6 in Western blot. In addition, a proportion of the sera recognized either a 59- or 70-kilodalton antigen, and 45% of the sera did not react in Western blot. Recently, the major LKM-3 antigen was identified as an autoepitope expressed on uridine diphosphate-glucuronosyltransferases (UGT). Seven LKM-3-positive sera reacted with recombinant rabbit family one UGT. None of the anti-LKM-1-positive hepatitis C sera reacted with UGT. Antibody reactivity against liver microsomal proteins in enzyme-linked immunosorbent assay ended when antigens were pretreated with sodium dodecyl sulfate, confirming that antibodies recognize conformational epitopes.

Conclusions: LKM-1 antibodies in hepatitis C are more heterogeneous and react with different antigens compared with LKM-3 antibodies in hepatitis D.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / blood
  • Antibody Specificity
  • Autoantibodies / blood*
  • Chronic Disease
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Hepatitis C / blood
  • Hepatitis C / immunology*
  • Hepatitis D / blood
  • Hepatitis D / immunology*
  • Humans
  • Immunoglobulin G / blood
  • Male
  • Rats


  • Antibodies, Anti-Idiotypic
  • Autoantibodies
  • Immunoglobulin G
  • anti-liver kidney microsome antibody