The long arm of human chromosome 14 (14q), an acrocentric chromosome, is estimated to be approximately 93 Mb in length. Genetic maps published to date have included mostly random DNA markers. The map we present here includes 13 genes, as well as 29 random DNA segments, based on typing of the CEPH families. Our map has been placed in context with other published maps. The resulting map of 42 loci has markers on average 5 cM apart and has a total length of 163 cM. The map order differs in the telomeric region from that of other maps previously published. 14q has homology, throughout approximately half its length, with mouse chromosome 12 and has a proximal region of homology with mouse chromosome 14.