Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Genomics. 1994 Sep 15;23(2):496-9. doi: 10.1006/geno.1994.1531.


Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Chromosomes, Artificial, Yeast
  • Chromosomes, Human, Pair 4*
  • DNA Primers / genetics
  • Glucuronosyltransferase / genetics*
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Multigene Family*
  • Polymerase Chain Reaction


  • DNA Primers
  • Glucuronosyltransferase