Putative functional domain within ORF2 on the Mycobacterium insertion sequences IS900 and IS902

Immunol Cell Biol. 1994 Oct;72(5):427-34. doi: 10.1038/icb.1994.63.


Repeated DNA sequences have been identified in a range of mycobacterial species and have been implicated in the increased virulence of some of these species, namely, Mycobacterium paratuberculosis and M. avium subsp. silvaticum. Here we present a case to suggest that the insertion sequences IS900 and IS902 encode a protein from a putative gene positioned on the complementary strand to their transposase genes. Based on amino acid homology analyses, this open reading frame (ORF2) could encode a transport protein. The ORF2 protein thus IS900 and IS902, may have a role in the increased pathogenicity of M. paratuberculosis and M. avium subsp. silvaticum from an M. avium background.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / analysis
  • Base Sequence
  • DNA Transposable Elements*
  • DNA, Bacterial / analysis
  • Molecular Sequence Data
  • Mycobacterium / genetics*
  • Mycobacterium / pathogenicity
  • Nucleotidyltransferases / genetics
  • Open Reading Frames*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transposases
  • Virulence


  • Bacterial Proteins
  • DNA Transposable Elements
  • DNA, Bacterial
  • Nucleotidyltransferases
  • Transposases

Associated data

  • GENBANK/S74401
  • GENBANK/S74415