Molecular cloning and structural characterization of the Arg-gingipain proteinase of Porphyromonas gingivalis. Biosynthesis as a proteinase-adhesin polyprotein

J Biol Chem. 1995 Jan 20;270(3):1007-10. doi: 10.1074/jbc.270.3.1007.


The identification of proteinases of Porphyromonas gingivalis that act as virulence factors in periodontal disease has important implications in the study of host-pathogen interactions as well as in the discovery of potential therapeutic and immunoprophylactic agents. We have cloned and characterized a gene that encodes the 50-kDa cysteine proteinase gingipain or Arg-gingipain-1 (RGP-1) described previously (Chen, Z., Potempa, J., Polanowski, A., Wikstrom, M., and Travis, J. (1992) J. Biol. Chem. 267, 18896-18901). Analysis of the amino acid sequence of RGP-1 deduced from the cloned DNA sequence showed that the biosynthesis of this proteinase involves processing of a polyprotein that contains multiple adhesin molecules located at its carboxyl terminus. This finding corroborates previous evidence (Pike R., McGraw, W., Potempa, J., and Travis, J. (1994) J. Biol. Chem. 269, 406-411) that RGP-1 is closely associated with adhesin molecules, and that high molecular weight forms of the proteinase are involved in the binding of erythrocytes.

MeSH terms

  • Adhesins, Bacterial
  • Amino Acid Sequence
  • Cell Adhesion Molecules / metabolism*
  • Cloning, Molecular
  • Cysteine Endopeptidases / biosynthesis
  • Cysteine Endopeptidases / chemistry
  • Cysteine Endopeptidases / genetics*
  • Gingipain Cysteine Endopeptidases
  • Hemagglutinins*
  • Molecular Sequence Data
  • Porphyromonas gingivalis / enzymology*
  • Protein Conformation
  • Sequence Homology, Amino Acid


  • Adhesins, Bacterial
  • Cell Adhesion Molecules
  • Gingipain Cysteine Endopeptidases
  • Hemagglutinins
  • Cysteine Endopeptidases

Associated data

  • GENBANK/U15282