AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier

J Biol Chem. 1995 Feb 3;270(5):2352-9. doi: 10.1074/jbc.270.5.2352.


We have characterized a new ankyrin gene, expressed in brain and other tissues, that is subject to extensive tissue-specific alternative mRNA processing. The full-length polypeptide has a molecular mass of 480 kDa and includes a predicted globular head domain, with membrane- and spectrin-binding activities, as well as an extended "tail" domain. We term this gene ankyrinG based on its giant size and general expression. Two brain-specific isoforms of 480 kDa and 270 kDa were identified that contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following the globular head domain. Antibodies against the serine-rich domain and spectrin-binding domain revealed labeling of nodes of Ranvier and axonal initial segments. Ankyrin-binding proteins also known to be localized in these specialized membrane domains include the voltage-dependent sodium channel, the sodium/potassium ATPase, sodium/calcium exchanger, and members of the neurofascin/L1 family of cell adhesion molecules. The neural-specific ankyrinG polypeptides are candidates to participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Ankyrins / genetics
  • Ankyrins / immunology
  • Ankyrins / metabolism*
  • Axons / ultrastructure
  • Base Sequence
  • Cerebral Cortex / chemistry
  • Fluorescent Antibody Technique
  • Gene Expression
  • Genes
  • Humans
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • RNA, Messenger / genetics
  • Ranvier's Nodes / chemistry*
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • Ankyrins
  • Nerve Tissue Proteins
  • RNA, Messenger

Associated data

  • GENBANK/U13616