DNA Binding Activities of the Caenorhabditis Elegans Tc3 Transposase

Nucleic Acids Res. 1994 Dec 25;22(25):5548-54. doi: 10.1093/nar/22.25.5548.

Abstract

Tc3 is a member of the Tc1/mariner family of transposable elements. All these elements have terminal inverted repeats, encode related transposases and insert exclusively into TA dinucleotides. We have studied the DNA binding properties of Tc3 transposase and found that an N-terminal domain of 65 amino acids binds specifically to two regions within the 462 bp Tc3 inverted repeat; one region is located at the end of the inverted repeat, the other is located approximately 180 bp from the end. Methylation interference experiments indicate that this N-terminal DNA binding domain of the Tc3 transposase interacts with nucleotides on one face of the DNA helix over adjacent major and minor grooves.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Caenorhabditis elegans / enzymology*
  • DNA / metabolism
  • DNA / ultrastructure
  • DNA Primers / chemistry
  • DNA-Binding Proteins / metabolism*
  • Molecular Sequence Data
  • Nucleotidyltransferases / metabolism*
  • Repetitive Sequences, Nucleic Acid
  • Transposases

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • DNA
  • Nucleotidyltransferases
  • Transposases