Loss of function mutation in the yeast multiple drug resistance gene PDR5 causes a reduction in chloramphenicol efflux

Antimicrob Agents Chemother. 1994 Oct;38(10):2492-4. doi: 10.1128/AAC.38.10.2492.


The yeast (Saccharomyces cerevisiae) PDR5 gene product encodes a 160-kDa protein related to the large ABC family of transporters, including the human MDR1 multidrug resistance p-glycoprotein. Loss of function mutations in PDR5 result in chloramphenicol hypersensitivity. A pdr5::Tn5 loss of function mutant exhibits a markedly impaired efflux of chloramphenicol compared with that of an isogenic PDR5 (wild-type) control.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Carrier Proteins / genetics*
  • Chloramphenicol / pharmacokinetics*
  • Drug Resistance, Multiple / genetics*
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Membrane Proteins / genetics*
  • Mutation
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*


  • ATP-Binding Cassette Transporters
  • Carrier Proteins
  • Fungal Proteins
  • Membrane Proteins
  • PDR5 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Chloramphenicol