Normal long bone growth and development in type X collagen-null mice

Nat Genet. 1994 Oct;8(2):129-35. doi: 10.1038/ng1094-129.


To investigate the role of type X collagen in skeletal development, we have generated type X collagen-null mice. Surprisingly, mice without type X collagen were viable and fertile and had no gross abnormalities in long bone growth or development. No differences were detected between the type X collagen-null mice and controls when growth plates of both newborn and 3-week old mice were examined by histology and by immunostaining for extracellular matrix components of bone including osteopontin, osteocalcin and type II collagen. Our results suggest that type X collagen is not required for long bone development. However, mice and humans with dominant acting type X collagen mutations have bone abnormalities, suggesting that only the presence of abnormal type X collagen can modify bone growth and development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Animals, Suckling
  • Base Sequence
  • Bone Development* / genetics
  • Cartilage / physiology
  • Collagen / classification
  • Collagen / deficiency*
  • Collagen / genetics
  • Extracellular Matrix / physiology
  • Growth Plate / chemistry
  • Growth Plate / ultrastructure
  • Humans
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Osteocalcin
  • Osteogenesis / genetics
  • Osteopontin
  • Sialoglycoproteins
  • Stem Cells


  • SPP1 protein, human
  • Sialoglycoproteins
  • Spp1 protein, mouse
  • Osteocalcin
  • Osteopontin
  • Collagen