Diversity of T cell receptors specific for the VSV antigenic peptide (N52-59) bound by the H-2Kb class I molecule

Cell Immunol. 1995 Jan;160(1):33-42. doi: 10.1016/0008-8749(95)80006-5.


As an approach to determine the structural basis of interactions between T cell receptors (TCRs) and MHC class I/peptide complexes, the fine specificities of a panel of vesicular stomatitis virus (VSV)-specific CTL clones recognizing the antigenic peptide (nucleoprotein 52-59) and the class I (Kb) molecule were correlated with the TCR primary structure. Each TCR showed a distinct interaction pattern with N52-59 and the Kb molecule. The large majority of the TCRs expressed by the panel of CTL clones used V beta 13 gene segments that had randomly recombined with D beta and J beta gene segments. The alpha chains were from randomly assorted V alpha and J alpha gene segments. Thus, the panel was found to be a highly heterogeneous set of TCRs, each member of which appeared to have an unique surface interface area, the recognition site, that interacted with a complementary surface formed by the single peptide bound in the class I antigenic groove.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Viral / immunology*
  • Base Sequence
  • Capsid / immunology
  • Clone Cells
  • H-2 Antigens / immunology*
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • Vesicular stomatitis Indiana virus / immunology*
  • Viral Core Proteins / immunology


  • Antigens, Viral
  • H-2 Antigens
  • H-2Kb protein, mouse
  • Receptors, Antigen, T-Cell, alpha-beta
  • Viral Core Proteins

Associated data

  • GENBANK/S76565
  • GENBANK/S76568
  • GENBANK/S76570
  • GENBANK/S76572
  • GENBANK/S76575
  • GENBANK/S76577