Analysis of the entire nucleotide sequence of the cryptic plasmid QpH1 from Coxiella burnetti

Eur J Epidemiol. 1994 Aug;10(4):413-20. doi: 10.1007/BF01719665.

Abstract

The complete plasmid QpH1 from Coxiella burnetti, isolate 'Nine Mile', phase I, was cloned as NotI fragment with a size of 37329 bp. The entire plasmid was sequenced by the chain termination method after EcoRI subcloning. 37 open reading frames coding for polypeptides larger than 100 amino acid residues were determined. The predicted polypeptide products of the open reading frames were compared by computer analysis with reported protein sequences. Homologies of predicted polypeptide products to analogous proteins are described.

MeSH terms

  • Amino Acids / genetics
  • Base Sequence
  • Cloning, Molecular
  • Codon / genetics
  • Coxiella burnetii / genetics*
  • DNA Primers
  • Escherichia coli / genetics
  • Genetic Vectors
  • Information Systems
  • Nucleotides / genetics*
  • Open Reading Frames
  • Peptide Chain Termination, Translational
  • Peptide Fragments / genetics
  • Peptides / genetics
  • Plasmids / genetics*
  • Protein Biosynthesis / genetics
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid

Substances

  • Amino Acids
  • Codon
  • DNA Primers
  • Nucleotides
  • Peptide Fragments
  • Peptides