Effect of beta-alanyl-L-histidinato zinc on protein components in osteoblastic MC3T3-El cells: increase in osteocalcin, insulin-like growth factor-I and transforming growth factor-beta

Mol Cell Biochem. 1994 Jul 27;136(2):163-9. doi: 10.1007/BF00926077.

Abstract

The effect of beta-alanyl-L-histidinato zinc (AHZ) on protein components in osteoblastic MC3T3-E1 cells was investigated. Cells were cultured for 3 days at 37 degrees C in CO2 incubator in plastic dishes containing alpha-modified minimum essential medium supplemented with 10% fetal bovine serum. After the cultures, the medium was exchanged for that containing 0.1% bovine serum albumin plus various concentrations of AHZ or other reagents, and the cells were cultured further 3 or 6 days. The homgenate of cells was analyzed with SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The presence of AHZ (10(-7) to 10(-5) M) caused an appreciable increase of many protein components in cells. Especially, the 67 killo-dalton (kDa) and 44 kDa proteins which are the major components from control cells were clearly increased by the presence of AHZ. Furthermore, the concentrations of osteocalcin, insulin-like growth factor-I and transforming growth factor-beta in the culture medium secreted from osteoblastic cells were markedly increased by the presence of AHZ (10(-6) and 10(-5) M). The effect of AHZ was a greater than that of zinc sulfate (10(-6) and 10(-5) M). The present findings suggest that AHZ can increase many proteins which are involved in the stimulation of bone formation and cell proliferation in osteoblastic cells.

MeSH terms

  • Carnosine* / analogs & derivatives*
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cell Line
  • Dipeptides / pharmacology*
  • Insulin-Like Growth Factor I / metabolism*
  • Organometallic Compounds / pharmacology*
  • Osteoblasts / drug effects*
  • Osteoblasts / metabolism
  • Osteocalcin / metabolism*
  • Stimulation, Chemical
  • Transforming Growth Factor beta / metabolism*
  • Zinc / pharmacology*
  • Zinc Compounds

Substances

  • Dipeptides
  • Organometallic Compounds
  • Transforming Growth Factor beta
  • Zinc Compounds
  • polaprezinc
  • Osteocalcin
  • Insulin-Like Growth Factor I
  • Carnosine
  • Zinc