Expression and sequence of outer surface protein C among North American isolates of Borrelia burgdorferi

FEMS Microbiol Lett. 1994 Dec 15;124(3):367-72. doi: 10.1111/j.1574-6968.1994.tb07310.x.


The expression of outer surface protein C (OspC) was determined for North American Borrelia burgdorferi isolates HB19, DN127cl9-2, 25015 and both low and high culture passage B31. A monoclonal antibody detected the presence of OspC protein in only two isolates, while polyclonal antiserum identified this protein in all five isolates. The ospC gene was cloned and sequenced for isolates HB19, DN127cl9-2 and 25015, and compared with the published ospC sequences of other Lyme disease spirochetes. Both the nucleotide and amino acid sequences were found to vary as much among isolates from the same geographic area as between isolates of different species.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antibodies
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Outer Membrane Proteins / immunology
  • Bacterial Outer Membrane Proteins / isolation & purification
  • Base Sequence
  • Borrelia burgdorferi Group / genetics*
  • Borrelia burgdorferi Group / immunology
  • Cloning, Molecular
  • DNA Probes
  • Humans
  • Immunoblotting
  • Molecular Sequence Data
  • North America


  • Antibodies
  • Bacterial Outer Membrane Proteins
  • DNA Probes

Associated data

  • GENBANK/U04280
  • GENBANK/U04281
  • GENBANK/U04282