Poly(vinyl alcohol) as a blocking agent in enzyme immunoassays

Immunol Invest. 1994 Nov;23(6-7):421-8. doi: 10.3109/08820139409066836.


We examined the blocking ability of poly(vinyl alcohol) (PVA) in enzyme immunoassays by coating polystyrene microtiter wells with PVA of different molecular weights (MW) and percent hydrolysis (%Hyd). Blocking ability was measured by the differences in non-specific binding of an anti-rabbit IgG-horseradish peroxidase conjugate to coated and uncoated wells. PVA with a MW of 124,000-186,000 and > 99 %Hyd was the most effective in suppressing the binding of the conjugate. This PVA at 0.5% (w/v) was significantly better at reducing non-specific binding than commonly used blocking agents and did not interfere with the specific binding of the conjugate to antigen-coated microtiter wells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Horseradish Peroxidase
  • Hydrolysis
  • Immunoenzyme Techniques*
  • Immunoglobulin G / analysis*
  • Molecular Weight
  • Polystyrenes / chemistry
  • Polyvinyl Alcohol / chemistry*
  • Proteins / chemistry
  • Rabbits


  • Immunoglobulin G
  • Polystyrenes
  • Proteins
  • Polyvinyl Alcohol
  • Horseradish Peroxidase