Molecular structure of a heme-copper redox center of the Escherichia coli ubiquinol oxidase: evidence and model

J Biochem. 1994 Sep;116(3):471-7. doi: 10.1093/oxfordjournals.jbchem.a124548.

Abstract

Biochemical, spectroscopic, and molecular biological studies on bacterial respiratory oxidases in the last decade have greatly increased our understanding of a molecular structure of the metal centers which catalyze the dioxygen reduction chemistry. Based upon the latest physicochemical and molecular biological evidence and theoretical consideration of a folding mechanics of membrane proteins, we propose here the tertiary structure of the heme-copper metal center of the Escherichia coli bo-type ubiquinol oxidase, the cytochrome bo complex. The molecular mechanism of electron transfer-coupled proton pumping in the heme-copper respiratory oxidases is reviewed on the basis of this predicted model.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Copper / chemistry*
  • Cytochrome b Group*
  • Cytochromes / chemistry*
  • Escherichia coli / enzymology*
  • Escherichia coli Proteins*
  • Heme / chemistry*
  • Models, Molecular*
  • Molecular Sequence Data
  • Molecular Structure
  • Oxidation-Reduction
  • Structure-Activity Relationship

Substances

  • Cytochrome b Group
  • Cytochromes
  • Escherichia coli Proteins
  • Heme
  • Copper
  • cytochrome bo, E coli