Mapping and sequence of the gene encoding protein p17, a major African swine fever virus structural protein

Virology. 1995 Feb 1;206(2):1140-4. doi: 10.1006/viro.1995.1039.


The gene encoding protein p17, a major structural protein of African swine fever virus, has been mapped and sequenced. Protein p17 was purified from dissociated virus by reverse-phase HPLC and the amino acid sequence of a peptide obtained after digestion of protein p17 with cyanogen bromide was determined by automated Edman degradation. To map the gene encoding protein p17, a mixture of 17-mer oligonucleotides based upon a part of the amino acid sequence was hybridized to cloned African swine fever virus DNA restriction fragments. This allowed the location of the gene in the fragment EcoRI D of the viral genome. The nucleotide sequence of a Sa/l/KpnI fragment revealed an open reading frame designated D117R encoding a protein of 117 amino acids with a deduced molecular mass of about 13,000 Da. A transcriptional analysis revealed that the p17 gene is expressed late in the viral infection cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Classical Swine Fever / virology
  • Classical Swine Fever Virus / genetics*
  • Classical Swine Fever Virus / metabolism
  • Cloning, Molecular
  • DNA Primers
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Viral*
  • Molecular Sequence Data
  • Molecular Weight
  • Open Reading Frames
  • Restriction Mapping
  • Swine
  • Transcription, Genetic
  • Viral Structural Proteins / analysis
  • Viral Structural Proteins / biosynthesis
  • Viral Structural Proteins / genetics*
  • Viral Structural Proteins / isolation & purification


  • DNA Primers
  • Viral Structural Proteins
  • p17 protein, African swine fever virus

Associated data

  • GENBANK/U15193
  • GENBANK/U18466