Regulation of c-fos expression in transgenic mice requires multiple interdependent transcription control elements

Neuron. 1995 Feb;14(2):241-52. doi: 10.1016/0896-6273(95)90282-1.


Transcription control regions of eukaryotic genes contain multiple sequence elements proposed to function independently to regulate transcription. We developed transgenic mice carrying fos-lacZ fusion genes with clustered point mutations in each of several distinct regulatory sequences: the sis-inducible element, the serum response element, the fos AP-1 site, and the calcium/cAMP response element. Analysis of Fos-lacZ expression in the CNS and in cultured cells demonstrated that all of the regulatory elements tested were required in concert for tissue- and stimulus-specific regulation of the c-fos promoter. This implies that the regulation of c-fos expression requires the concerted action of multiple control elements that direct the assembly of an interdependent transcription complex.

MeSH terms

  • Animals
  • Binding Sites
  • Cloning, Molecular
  • Embryo, Mammalian
  • Gene Expression
  • Gene Expression Regulation*
  • Genes, fos*
  • Glial Fibrillary Acidic Protein / analysis
  • Kainic Acid
  • Mice
  • Mice, Transgenic
  • Microtubule-Associated Proteins / analysis
  • Mutagenesis, Site-Directed
  • Neurons / drug effects
  • Neurons / metabolism
  • Neurons / physiology
  • Point Mutation
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid*
  • Seizures / chemically induced
  • Seizures / physiopathology
  • Telencephalon / physiology
  • Transcription Factor AP-1 / metabolism
  • Transcription, Genetic
  • beta-Galactosidase / biosynthesis


  • Glial Fibrillary Acidic Protein
  • Microtubule-Associated Proteins
  • Transcription Factor AP-1
  • beta-Galactosidase
  • Kainic Acid