Multiple protein kinases are required for basal Kv1.5 K+ channel gene expression in GH3 clonal pituitary cells

Biochim Biophys Acta. 1995 Feb 16;1265(1):22-8. doi: 10.1016/0167-4889(94)00205-s.


The role of protein kinases in maintaining basal expression of voltage-gated K+ channel mRNA was examined in GH3 clonal pituitary cells. Nonspecific inhibition of protein kinases with H7 or staurosporine markedly decreases Kv1.5 K+ channel gene transcription and mRNA without producing a substantial change in Kv1.4 mRNA. Selective inhibitors for protein kinase C, Ca(2+)-calmodulin kinases, and tyrosine kinases do not affect Kv1.5 mRNA expression. In contrast, the Rp-diastereomer of adenosine 3',5'-cyclic monophosphorothioate, a specific inhibitor of protein kinase A, partially inhibits Kv1.5 mRNA expression (approximately 40%), and this effect was antagonized by 8-bromo-adenosine 3',5'-cyclic monophosphate. Thus, protein kinase A and at least one other kinase are required for basal Kv1.5 mRNA expression in pituitary cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Alkaloids / pharmacology
  • Animals
  • Clone Cells
  • Gene Expression
  • Isoquinolines / pharmacology
  • Piperazines / pharmacology
  • Pituitary Gland / metabolism*
  • Potassium Channels / genetics*
  • Protein Kinase Inhibitors*
  • RNA, Messenger / metabolism
  • Rats
  • Staurosporine
  • Transcription, Genetic / drug effects


  • Alkaloids
  • Isoquinolines
  • Piperazines
  • Potassium Channels
  • Protein Kinase Inhibitors
  • RNA, Messenger
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Staurosporine