Pharmacokinetics of hydroxyurea in nude mice

Anticancer Drugs. 1994 Oct;5(5):573-8. doi: 10.1097/00001813-199410000-00009.


Extrachromosomal DNA is the predominant form of gene amplification in human tumors. Hydroxyurea (HU) concentrations of 100-150 microM have been promising in vitro for extrachromosomal DNA elimination. The study objective was to determine the HU dose-concentration relationship in nude mice with HU doses from 0 to 200 mg/kg. For HU t1/2 determination, mice were injected with HU 100 mg/kg. A plasma concentration of 159 microM was achieved and a t1/2 of 11.3 min determined. Based on these findings, In vivo elimination studies will require frequent administration of HU to maintain plasma concentrations from 100 to 150 microM.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cells, Cultured
  • Culture Media
  • DNA, Neoplasm / biosynthesis
  • DNA, Neoplasm / drug effects*
  • Half-Life
  • Hydroxyurea / administration & dosage
  • Hydroxyurea / pharmacokinetics*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Tissue Distribution


  • Culture Media
  • DNA, Neoplasm
  • Hydroxyurea