Coupled-column high-performance liquid chromatographic method for the determination of four metabolites of polycyclic aromatic hydrocarbons, 1-, 4- and 9-hydroxyphenanthrene and 1-hydroxypyrene, in urine

J Chromatogr B Biomed Appl. 1994 Oct 3;660(1):67-73. doi: 10.1016/0378-4347(94)00267-3.


A coupled-column high-performance liquid chromatographic method applying system-integrated sample processing on a copper phthalocyanine modified solid-phase material, gradient elution and fluorescence detection for the quantification of 1-, 4- and 9-hydroxyphenanthrene as well as for 1-hydroxypyrene in urine is described. The automated chromatographic system tolerates direct and repeated injection of urine samples. Validation results presented indicate quantitative and matrix-independent recoveries, low imprecisions in within-series and between-series analysis in combination with broad linear working ranges (2-400 nmol/l) and low detection limits (< 0.1 nmol/l).

MeSH terms

  • Chromatography, High Pressure Liquid
  • Humans
  • Isomerism
  • Phenanthrenes / urine
  • Polycyclic Compounds / urine*
  • Pyrenes / analysis


  • Phenanthrenes
  • Polycyclic Compounds
  • Pyrenes
  • 1-hydroxyphenanthrene
  • 4-hydroxyphenanthrene
  • 9-phenanthrol
  • 1-hydroxypyrene